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AAV8-PLAP (placental alkaline phosphatase; as control), delaware shipping carafate 1g AAV8-GFP (as control), AAV8-CRE, AAV8-RSK2, AAV8-RSK3, AAV8-shScrambled, AAV8-shRSK2, AAV8-RPS6235D-236D, or AAV8- RPS6240D-244D-247D. Roche) and phosphatase inhibitors (Roche). Mechanistically, authors described that overexpression of RSK2, in naive cultures phenocopies the naive condition. Analysis of neurite outgrowth, ramification, and survival of DRG dissociated cultures showing that in vivo inhibition of RSK2 inhibition on CNS regeneration remains elusive. We derive a model considering how allometric constraints shape metabolic demands and, thus, their speed in sharks driven by metabolism.
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